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Bioinformatics Advance Access published online on April 8, 2004
Bioinformatics, doi:10.1093/bioinformatics/bth254
Bioinformatics © Oxford University Press 2004; all rights reserved
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Received December 17, 2003
Revised March 9, 2004
Accepted March 17, 2004

Article

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR

Owen J. Marshall 1*

1 Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Rd., Parkville, Vic, 3052, Australia

* To whom correspondence should be addressed. E-mail: owen.marshall{at}mcri.edu.au.


  Abstract

Summary: PerlPrimer is a cross-platform GUI application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.

Availability: Open-source and freely available from http://perlprimer.sourceforge.net.


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