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Bioinformatics Advance Access published online on April 8, 2004

Bioinformatics, doi:10.1093/bioinformatics/bth259
Bioinformatics © Oxford University Press 2004; all rights reserved
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Received January 25, 2004
Revised February 27, 2004
Accepted March 9, 2004

Applications note

G-PRIMER: greedy algorithm for selecting minimal primer set

Jiren Wang 1*, Kuo-Bin Li 1, Wing-Kin Sung 2

1 BioInformatics Institute, 30 Biopolis Street, #07-01 Matrix, Singapore 138671
2 Department of Computer Science, National University of Singapore, 3 Science Drive 2, Singapore 117543

* To whom correspondence should be addressed. E-mail: jiren{at}bii.a-star.edu.sg.


   Abstract

Summary: G-PRIMER, a web-based primer design program, has been developed to compute a minimal primer set (MPS) specifically annealed to all of the open reading frames (ORFs) in a given microbial genome. This program has been successfully used in the microarray experiment for analyzing the expression of genes in the Xanthomonas Campestris genome.

Availability: It is available at http://mammoth.bii.a-star.edu.sg/gprimer/. Its source code is available upon request.


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