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Bioinformatics Advance Access published online on August 12, 2004
Bioinformatics, doi:10.1093/bioinformatics/bth472
Bioinformatics © Oxford University Press 2004; all rights reserved
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Received May 9, 2004
Revised July 21, 2004
Accepted August 3, 2004

Article

A rapid method for computationally inferring transcriptome coverage and microarray sensitivity

A. Reverter 1*, S. M. McWilliam 1, W. Barris 1, B. P. Dalrymple 1

1 Bioinformatics Group, CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Rd., St. Lucia, QLD 4067, Australia

* To whom correspondence should be addressed. E-mail: Tony.Reverter-Gomez{at}csiro.au.


  Abstract

Motivation: There are many different gene expression technologies, including cDNA and oligo-based microarrays, SAGE and MPSS. For each organism of interest, coverage of the transcriptome and the genome will be different. We address the question of what level of coverage is required to exploit the sensitivity of the different technologies, and what is the sensitivity of the different approaches in the experimental study.

Results: We estimate transcriptome coverage by randomly sampling transcripts from a pre-defined tag-to-gene mapping function. For a given microarray experiment, we locate the thresholds in intensities that define the distribution of transcript abundance. These values are compared against the distribution obtained by applying the same thresholds to the intensities from differentially expressed genes. The ratio of these two distributions meets at the equilibrium defining sensitivity. We conclude that a collection of ~340,000 sequences is adequate for microarrays, but not large enough for maximum utilisation of tag-based technologies. In the absence of large-scale sequencing, the majority of the tags detected by the latter approaches will remain unidentified until the genome sequence is available.


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