Skip Navigation



Bioinformatics Advance Access published online on December 14, 2004
Bioinformatics, doi:10.1093/bioinformatics/bti219
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
21/8/1701    most recent
bti219v1
Right arrow Comments: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Comments are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kaplinski, L.
Right arrow Articles by Remm, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaplinski, L.
Right arrow Articles by Remm, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Bioinformatics © Oxford University Press 2004; all rights reserved.
Received October 11, 2004
Revised November 25, 2004
Accepted December 10, 2004

Applications note

MultiPLX: automatic grouping and evaluation of PCR primers

Lauris Kaplinski 1, Reidar Andreson 2, Tarmo Puurand 2, and Maido Remm 2*

1 Department of Bioinformatics, University of Tartu; Estonian Biocentre
2 Department of Bioinformatics, University of Tartu

* To whom correspondence should be addressed.
Maido Remm, E-mail: maido.remm{at}ut.ee


  Abstract

Summary: MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer-primer interactions, primer-product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. An unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs.

Availability: Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
R. Andreson, T. Mols, and M. Remm
Predicting failure rate of PCR in large genomes
Nucleic Acids Res., June 1, 2008; 36(11): e66 - e66.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.