Skip Navigation



Bioinformatics Advance Access published online on July 27, 2007
Bioinformatics, doi:10.1093/bioinformatics/btm381
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow Supplementary data
Right arrow All Versions of this Article:
23/19/2543    most recent
btm381v1
Right arrow Comments: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Comments are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Zucko, J.
Right arrow Articles by Hranueli, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zucko, J.
Right arrow Articles by Hranueli, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author (2007). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Polyketide synthase genes and the natural products potential of Dictyostelium discoideum

J. Zucko 1,5, N. Skunca 1, T. Curk 2, B. Zupan 2,3, P.F. Long 4, J. Cullum 5, R.H. Kessin 6 and D. Hranueli 1,*

1Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia, 2Faculty of Computer and Information Science, University of Ljubljana, Trzaska cesta 25, SI-1001 Ljubljana, Slovenia, 3Department of Molecular and Human Genetics, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA, 4The School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, UK, 5Department of Genetics, University of Kaiserslautern, Postfach 3049, 67653 Kaiserslautern, Germany, 6Department of Anatomy and Cell Biology, Columbia University, 630 West 168th St., 10032 New York, USA

*To whom correspondence should be addressed. D. Hranueli, E-mail: dhranueli{at}pbf.hr


  Abstract

Motivation: The genome of the social amoeba Dictyostelium discoideum contains an unusually large number of polyketide synthase (PKS) genes. An analysis of the genes is a first step towards understanding the biological roles of their products and exploiting novel products.

Results: 45 Type I iterative PKS genes were found, 5 of which are probably pseudogenes. Catalytic domains that are homologous with known PKS sequences as well as possible novel domains were identified. The genes often occurred in clusters of 2-5 genes, where members of the cluster had very similar sequences. The D. discoideum PKS genes formed a clade distinct from fungal and bacterial genes. All nine genes examined by RT-PCR were expressed, although at different developmental stages. The promoters of PKS genes were much more divergent than the structural genes, although we have identified motifs that are unique to some PKS gene promoters.

Availability:

Contact: dhranueli{at}pbf.hr

Supplementary information: Supplementary data are available at Bioinformatics online.

Associate Editor: Prof. Dmitrij Frishman

Received on May 15, 2007; revised on July 9, 2007; accepted on July 10, 2007

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Starcevic, J. Zucko, J. Simunkovic, P. F. Long, J. Cullum, and D. Hranueli
ClustScan: an integrated program package for the semi-automatic annotation of modular biosynthetic gene clusters and in silico prediction of novel chemical structures
Nucleic Acids Res., December 1, 2008; 36(21): 6882 - 6892.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.