Skip Navigation



Bioinformatics Advance Access published online on January 28, 2009
Bioinformatics, doi:10.1093/bioinformatics/btn663
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrowOA All Versions of this Article:
25/5/650    most recent
btn663v2
btn663v1
Right arrow Comments: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Comments are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Zhang, L.
Right arrow Articles by Coombes, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, L.
Right arrow Articles by Coombes, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Serial dilution curve: a new method for analysis of reverse phase protein array data

Li Zhang 1,*, Qingyi Wei 2, Li Mao 3, Wenbin Liu 1,4, Gordon B. Mills 4 and Kevin Coombes 1

1Department of Bioinformatics and Computational Biology; 2Department of Epidemiology; 3Department of Thoracic and Head and Neck Medical Oncology; 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center. 1400 Pressler street, unit 1410, Houston, Texas 77030, USA.

*To whom correspondence should be addressed. Dr. Li Zhang, E-mail: lzhangli{at}mdanderson.org


  Abstract

Reverse phase protein arrays (RPPA) are a powerful high throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dynamic range of the measurements and to increase confidence in quantitation. An RPPA experiment is equivalent to running multiple ELISA assays concurrently except that there usually is no known protein concentration from which one can construct a standard re-sponse curve. Here we describe a new method called "serial dilution curve for RPPA data analysis". Compared with the existing methods, the new method has the advantage of using fewer parameters and offering a simple way of visualizing the raw data. We showed how the method can be used to examine data quality and to obtain ro-bust quantification of protein concentrations.

Availability: A computer program in R for using serial dilution curve for RPPA data analysis is freely available at: url=http://odin.mdacc.tmc.edu/~zhangli/RPPA.

Associate Editor: Dr. Jonathan Wren

Received on April 25, 2008; revised on December 3, 2008; accepted on December 27, 2008

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BioinformaticsHome page
E. S. Neeley, S. M. Kornblau, K. R. Coombes, and K. A. Baggerly
Variable slope normalization of reverse phase protein arrays
Bioinformatics, June 1, 2009; 25(11): 1384 - 1389.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.