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Bioinformatics Advance Access published online on February 2, 2009
Bioinformatics, doi:10.1093/bioinformatics/btp046
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© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

VANO: A Volume-Object Image Annotation System

Hanchuan Peng , Fuhui Long and Eugene W. Myers

Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. Email: pengh{at}janelia.hhmi.org

*To whom correspondence should be addressed. Hanchuan Peng, E-mail: pengh{at}janelia.hhmi.org


  Abstract

VANO is a cross-platform image annotation system that enables one to conveniently visualize and annotate 3D volume objects including nuclei and cells. An application of VANO typically starts with an initial collection of objects produced by a segmentation computation. The objects can then be labeled, categorized, deleted, added, split, merged, and redefined. VANO has been used to build high-resolution digital atlases of the nuclei of C. elegans at the L1 stage and the nuclei of D. melanogaster's ventral nerve cord at the late embryonic stage.

Availability: Platform independent executables of VANO, a sample dataset, and a detailed description of both its design and usage are available at: research.janelia.org/peng/proj/vano. VANO is open-source for co-development.

Associate Editor: Dr. Jonathan Wren

Received on December 9, 2008; revised on January 14, 2009; accepted on January 16, 2009

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